Review



pcr-based mirna microarray platform  (Qiagen)


Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Qiagen pcr-based mirna microarray platform
    Total RNA was isolated from WT and IL10KO mice BM-FPCs using miRNeasy kit (Qiagen) and fibrosis-associated <t>miRNA</t> platform was used to assess the miRNAs level using Taqman microRNA assay. (A) At basal level fibrosis-associated miRNAs expression was significantly increased in IL10KO BM-FPCs compared to WT BM-FPCs with maximum expression of miRNA21, 145 and 208a (N=6). (B–D) miRNA21/145/208a expression was measured in WT-FPCs treated with IL10 and TGFβ by RT-PCR. IL10 treatment significantly inhibits TGFβ-induced miRNA-21, -145 and 208a expression. **p<0.01 vs BSA, #p<0.05 vs TGFβ. IL10KO mice: IL10 Knockout mice; BM-FPC: Bone marrow fibroblast progenitor cells. (N=6)
    Pcr Based Mirna Microarray Platform, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr-based mirna microarray platform/product/Qiagen
    Average 90 stars, based on 1 article reviews
    pcr-based mirna microarray platform - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Interleukin 10 Inhibits Bone Marrow Fibroblast Progenitor Cell-mediated Cardiac Fibrosis in Pressure Overloaded Myocardium"

    Article Title: Interleukin 10 Inhibits Bone Marrow Fibroblast Progenitor Cell-mediated Cardiac Fibrosis in Pressure Overloaded Myocardium

    Journal: Circulation

    doi: 10.1161/CIRCULATIONAHA.117.027889

    Total RNA was isolated from WT and IL10KO mice BM-FPCs using miRNeasy kit (Qiagen) and fibrosis-associated miRNA platform was used to assess the miRNAs level using Taqman microRNA assay. (A) At basal level fibrosis-associated miRNAs expression was significantly increased in IL10KO BM-FPCs compared to WT BM-FPCs with maximum expression of miRNA21, 145 and 208a (N=6). (B–D) miRNA21/145/208a expression was measured in WT-FPCs treated with IL10 and TGFβ by RT-PCR. IL10 treatment significantly inhibits TGFβ-induced miRNA-21, -145 and 208a expression. **p<0.01 vs BSA, #p<0.05 vs TGFβ. IL10KO mice: IL10 Knockout mice; BM-FPC: Bone marrow fibroblast progenitor cells. (N=6)
    Figure Legend Snippet: Total RNA was isolated from WT and IL10KO mice BM-FPCs using miRNeasy kit (Qiagen) and fibrosis-associated miRNA platform was used to assess the miRNAs level using Taqman microRNA assay. (A) At basal level fibrosis-associated miRNAs expression was significantly increased in IL10KO BM-FPCs compared to WT BM-FPCs with maximum expression of miRNA21, 145 and 208a (N=6). (B–D) miRNA21/145/208a expression was measured in WT-FPCs treated with IL10 and TGFβ by RT-PCR. IL10 treatment significantly inhibits TGFβ-induced miRNA-21, -145 and 208a expression. **p<0.01 vs BSA, #p<0.05 vs TGFβ. IL10KO mice: IL10 Knockout mice; BM-FPC: Bone marrow fibroblast progenitor cells. (N=6)

    Techniques Used: Isolation, TaqMan microRNA Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Knock-Out

    BM-FPCs were transfected with miRNA-21 mimic for 12 hours using lipofectamine 2000 transfection reagent before IL10 and TGFβ treatments and miRNA-21 expression was measured by RT-PCR. (A) miRNA-21 mimic transfection significantly increased miRNA-21 expression in BM-FPCs. (B–E) 24 hrs after mimic, IL10 and TGFβ treatments, cells were harvested to measured the expression of fibrosis marker genes by RT-PCR. Relative levels of target genes expression were normalized with 18S. IL10 significantly inhibited TGFβ-induced fibrosis-associated genes expression. Restoration of miRNA-21 expression using miRNA-21 mimic significantly attenuated the IL10 effect on expression of these genes. ***p<0.001, **p<0.01, *p<0.05 vs BSA, ###p<0.001, ##p<0.01 vs TGFβ; $$$p<0.001, $p<0.05 vs TGFβ and ^^^p<0.001, ^^p<0.01, ^p<0.05 vs miR-21 mimic alone. (N=6) (F) Schematic diagram indicates TAC-induced mobilization and homing of BM-FPCs to the heart. In the heart, profibrotic stimuli (TGFβ in this case) first facilitates BM-FPC activation to myofibroblasts and then promote Smad2/3 dependent miRNA-21 maturation. The miRNA-21 maturation ultimately leads to fibrotic gene activation in activated myofibroblasts. On the other hand, IL10 inhibits TAC-induced mobilization, homing and activation of these cells and regulates cardiac fibrosis. Factor: Chemokine/cytokines; BMCs: Bone marrow cells; IL10 Interleukin-10.
    Figure Legend Snippet: BM-FPCs were transfected with miRNA-21 mimic for 12 hours using lipofectamine 2000 transfection reagent before IL10 and TGFβ treatments and miRNA-21 expression was measured by RT-PCR. (A) miRNA-21 mimic transfection significantly increased miRNA-21 expression in BM-FPCs. (B–E) 24 hrs after mimic, IL10 and TGFβ treatments, cells were harvested to measured the expression of fibrosis marker genes by RT-PCR. Relative levels of target genes expression were normalized with 18S. IL10 significantly inhibited TGFβ-induced fibrosis-associated genes expression. Restoration of miRNA-21 expression using miRNA-21 mimic significantly attenuated the IL10 effect on expression of these genes. ***p<0.001, **p<0.01, *p<0.05 vs BSA, ###p<0.001, ##p<0.01 vs TGFβ; $$$p<0.001, $p<0.05 vs TGFβ and ^^^p<0.001, ^^p<0.01, ^p<0.05 vs miR-21 mimic alone. (N=6) (F) Schematic diagram indicates TAC-induced mobilization and homing of BM-FPCs to the heart. In the heart, profibrotic stimuli (TGFβ in this case) first facilitates BM-FPC activation to myofibroblasts and then promote Smad2/3 dependent miRNA-21 maturation. The miRNA-21 maturation ultimately leads to fibrotic gene activation in activated myofibroblasts. On the other hand, IL10 inhibits TAC-induced mobilization, homing and activation of these cells and regulates cardiac fibrosis. Factor: Chemokine/cytokines; BMCs: Bone marrow cells; IL10 Interleukin-10.

    Techniques Used: Transfection, Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Activation Assay



    Similar Products

    pcr-based mirna microarray platform  (Qiagen)
    90
    Qiagen pcr-based mirna microarray platform
    Total RNA was isolated from WT and IL10KO mice BM-FPCs using miRNeasy kit (Qiagen) and fibrosis-associated <t>miRNA</t> platform was used to assess the miRNAs level using Taqman microRNA assay. (A) At basal level fibrosis-associated miRNAs expression was significantly increased in IL10KO BM-FPCs compared to WT BM-FPCs with maximum expression of miRNA21, 145 and 208a (N=6). (B–D) miRNA21/145/208a expression was measured in WT-FPCs treated with IL10 and TGFβ by RT-PCR. IL10 treatment significantly inhibits TGFβ-induced miRNA-21, -145 and 208a expression. **p<0.01 vs BSA, #p<0.05 vs TGFβ. IL10KO mice: IL10 Knockout mice; BM-FPC: Bone marrow fibroblast progenitor cells. (N=6)
    Pcr Based Mirna Microarray Platform, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr-based mirna microarray platform/product/Qiagen
    Average 90 stars, based on 1 article reviews
    pcr-based mirna microarray platform - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Total RNA was isolated from WT and IL10KO mice BM-FPCs using miRNeasy kit (Qiagen) and fibrosis-associated miRNA platform was used to assess the miRNAs level using Taqman microRNA assay. (A) At basal level fibrosis-associated miRNAs expression was significantly increased in IL10KO BM-FPCs compared to WT BM-FPCs with maximum expression of miRNA21, 145 and 208a (N=6). (B–D) miRNA21/145/208a expression was measured in WT-FPCs treated with IL10 and TGFβ by RT-PCR. IL10 treatment significantly inhibits TGFβ-induced miRNA-21, -145 and 208a expression. **p<0.01 vs BSA, #p<0.05 vs TGFβ. IL10KO mice: IL10 Knockout mice; BM-FPC: Bone marrow fibroblast progenitor cells. (N=6)

    Journal: Circulation

    Article Title: Interleukin 10 Inhibits Bone Marrow Fibroblast Progenitor Cell-mediated Cardiac Fibrosis in Pressure Overloaded Myocardium

    doi: 10.1161/CIRCULATIONAHA.117.027889

    Figure Lengend Snippet: Total RNA was isolated from WT and IL10KO mice BM-FPCs using miRNeasy kit (Qiagen) and fibrosis-associated miRNA platform was used to assess the miRNAs level using Taqman microRNA assay. (A) At basal level fibrosis-associated miRNAs expression was significantly increased in IL10KO BM-FPCs compared to WT BM-FPCs with maximum expression of miRNA21, 145 and 208a (N=6). (B–D) miRNA21/145/208a expression was measured in WT-FPCs treated with IL10 and TGFβ by RT-PCR. IL10 treatment significantly inhibits TGFβ-induced miRNA-21, -145 and 208a expression. **p<0.01 vs BSA, #p<0.05 vs TGFβ. IL10KO mice: IL10 Knockout mice; BM-FPC: Bone marrow fibroblast progenitor cells. (N=6)

    Article Snippet: Fibrosis-associated miRNA expression was analyzed in WT and IL10 KO BM-FPCs at basal level using a PCR-based miRNA microarray platform (Qiagen). miRNA-array data showed that several miRNAs were differentially expressed in IL10KO BM-FPCs compared to WT BM-FPCs ( ).

    Techniques: Isolation, TaqMan microRNA Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Knock-Out

    BM-FPCs were transfected with miRNA-21 mimic for 12 hours using lipofectamine 2000 transfection reagent before IL10 and TGFβ treatments and miRNA-21 expression was measured by RT-PCR. (A) miRNA-21 mimic transfection significantly increased miRNA-21 expression in BM-FPCs. (B–E) 24 hrs after mimic, IL10 and TGFβ treatments, cells were harvested to measured the expression of fibrosis marker genes by RT-PCR. Relative levels of target genes expression were normalized with 18S. IL10 significantly inhibited TGFβ-induced fibrosis-associated genes expression. Restoration of miRNA-21 expression using miRNA-21 mimic significantly attenuated the IL10 effect on expression of these genes. ***p<0.001, **p<0.01, *p<0.05 vs BSA, ###p<0.001, ##p<0.01 vs TGFβ; $$$p<0.001, $p<0.05 vs TGFβ and ^^^p<0.001, ^^p<0.01, ^p<0.05 vs miR-21 mimic alone. (N=6) (F) Schematic diagram indicates TAC-induced mobilization and homing of BM-FPCs to the heart. In the heart, profibrotic stimuli (TGFβ in this case) first facilitates BM-FPC activation to myofibroblasts and then promote Smad2/3 dependent miRNA-21 maturation. The miRNA-21 maturation ultimately leads to fibrotic gene activation in activated myofibroblasts. On the other hand, IL10 inhibits TAC-induced mobilization, homing and activation of these cells and regulates cardiac fibrosis. Factor: Chemokine/cytokines; BMCs: Bone marrow cells; IL10 Interleukin-10.

    Journal: Circulation

    Article Title: Interleukin 10 Inhibits Bone Marrow Fibroblast Progenitor Cell-mediated Cardiac Fibrosis in Pressure Overloaded Myocardium

    doi: 10.1161/CIRCULATIONAHA.117.027889

    Figure Lengend Snippet: BM-FPCs were transfected with miRNA-21 mimic for 12 hours using lipofectamine 2000 transfection reagent before IL10 and TGFβ treatments and miRNA-21 expression was measured by RT-PCR. (A) miRNA-21 mimic transfection significantly increased miRNA-21 expression in BM-FPCs. (B–E) 24 hrs after mimic, IL10 and TGFβ treatments, cells were harvested to measured the expression of fibrosis marker genes by RT-PCR. Relative levels of target genes expression were normalized with 18S. IL10 significantly inhibited TGFβ-induced fibrosis-associated genes expression. Restoration of miRNA-21 expression using miRNA-21 mimic significantly attenuated the IL10 effect on expression of these genes. ***p<0.001, **p<0.01, *p<0.05 vs BSA, ###p<0.001, ##p<0.01 vs TGFβ; $$$p<0.001, $p<0.05 vs TGFβ and ^^^p<0.001, ^^p<0.01, ^p<0.05 vs miR-21 mimic alone. (N=6) (F) Schematic diagram indicates TAC-induced mobilization and homing of BM-FPCs to the heart. In the heart, profibrotic stimuli (TGFβ in this case) first facilitates BM-FPC activation to myofibroblasts and then promote Smad2/3 dependent miRNA-21 maturation. The miRNA-21 maturation ultimately leads to fibrotic gene activation in activated myofibroblasts. On the other hand, IL10 inhibits TAC-induced mobilization, homing and activation of these cells and regulates cardiac fibrosis. Factor: Chemokine/cytokines; BMCs: Bone marrow cells; IL10 Interleukin-10.

    Article Snippet: Fibrosis-associated miRNA expression was analyzed in WT and IL10 KO BM-FPCs at basal level using a PCR-based miRNA microarray platform (Qiagen). miRNA-array data showed that several miRNAs were differentially expressed in IL10KO BM-FPCs compared to WT BM-FPCs ( ).

    Techniques: Transfection, Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Activation Assay